Somnath Ghosh » Публикация
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Опубликовано
2010-08-04
Опубликовано на SciPeople2012-06-18 09:54:40
ЖурналCarbohydrate Research 345 (2010) 2220–2227
Antimicrobial activity of highly stable silver nanoparticles embedded in agar–agar matrix as a thin film
Аннотация
Highly stable silver nanoparticles (Ag NPs) in agar–agar (Ag/agar) as inorganic–organic hybrid were
obtained as free-standing film by in situ reduction of silver nitrate by ethanol. The antimicrobial activity
of Ag/agar film on Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Candida albicans (C. albicans)
was evaluated in a nutrient broth and also in saline solution. In particular, films were repeatedly
tested for antimicrobial activity after recycling. UV–vis absorption and TEM studies were carried out
on films at different stages and morphological studies on microbes were carried out by SEM. Results
showed spherical Ag NPs of size 15–25 nm, having sharp surface plasmon resonance (SPR) band. The antimicrobial
activity of Ag/agar film was found to be in the order, C. albicans > E. coli > S. aureus, and antimicrobial
activity against C. albicans was almost maintained even after the third cycle. Whereas, in case of
E. coli and S. aureus there was a sharp decline in antimicrobial activity after the second cycle. Agglomeration
of Ag NPs in Ag/agar film on exposure to microbes was observed by TEM studies. Cytotoxic experiments
carried out on HeLa cells showed a threshold Ag NPs concentration of 60 lg/mL, much higher than
the minimum inhibition concentration of Ag NPs (25.8 lg/mL) for E. coli. The mechanical strength of the
film determined by nanoindentation technique showed almost retention of the strength even after
repeated cycle.
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