Venkataramanappa YARRADODDAPPA » Публикация
Поделиться публикацией:
Опубликовано
2010-04-00
Опубликовано на SciPeople2012-12-04 11:10:20
ЖурналFood Anal. Methods (2011) 4:90–99
Quantification of Nitrite/Nitrate in Food Stuff Samples Using 2-Aminobenzoic Acid as a New Amine in Diazocoupling Reaction
Аннотация
2-Aminobenzoic acid has been used as an amine
in diazocoupling reaction to form an azo dye in the
quantification of nitrite/nitrate at trace level. The formed
azo dye has an absorption maximum at 550 nm in aqueous
phase, and the resulted dye can be extracted into organic
solvent to lower the detection limit. The method obeys
Beer's law in the concentration range 0–10 μg of nitrite in
25 ml of aqueous solution with a molar absorptivity of
3.6×103 L mol−
1cm−1 and 0–2 μg of nitrite in 5 ml of
organic phase. The detection limit of the dye has been
found to be 0.056 μg ml−
1. Nitrate is determined by
reducing it to nitrite after passing through a copperized
cadmium reductor column. The effect of interfering ions on
the determination of nitrite/nitrate has been described. The
developed method has been applied to determine the
nitrite/nitrate trace level in vegetable, fruit juice, and milk
powder samples.
Комментарии
Вам необходимо зайти или зарегистрироваться для комментирования
Этот комментарий был удален
Этот комментарий был удален