Алексей (alexei) Некрасов (nekrasov) » Публикация
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Опубликовано
2008-00-00
Опубликовано на SciPeople2014-06-27 21:50:54
ЖурналJournal of Biomolecular Structure & Dynamics
Hydrolases: The Correlation Between Informational Structure and the Catalytic Sites Organization
Journal of Biomolecular Structure &
Dynamics, ISSN 0739-1102
Volume 25, Issue Number 5, (2008) pp. 553-561
Аннотация
The novel method allowing identification of protein structure elements responsible for catalytic
activity manifestation is proposed. Structural organization of various hydrolases was
studied using the ANIS (ANalysis of Informational Structure) method. ANIS allows to
reveal a hierarchy of the ELements of Information Structure (ELIS) using protein amino
acid sequence. The ELIS corresponds to the variable length sites with an increased density
of structural information. The amino acid residues forming the enzyme catalytic site were
shown to belong to the different top-ranking ELIS located in the contact area of the corresponding
spatial structure clusters. In the protein spatial structure catalytic sites are located
in the area of contact between fragments of polypeptide chain (structural blocs) allocation
to the different top-ranking ELIS. According to our results we concluded that structural
blocks corresponding to top-ranking ELIS are crucial for protein functioning. Such regions
are structurally independent, and their determinate mobility relative to each other is vital for
an efficient enzymatic reaction to occur.