Алексей (alexei) Некрасов (nekrasov) » Публикация

Аннотация Interleukin
13 (IL
13) is one of the cytokines involved in the development of Th2
type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL
13 were constructed on a basis of informational structure analysis and expressed in E. coli cells. They were found to differ in ability to stimulate proliferation of TF
1 cell line. Deletion variant 146 (DV146) completely lacks such activity, whereas DV148 provides about 50% of the proliferation stimulation. The simultaneous addition of DV146 with full
length IL
13 suppresses proliferation depending on the concentration of the deletion form. Thus, the designed protein acts as an antagonist of IL
13.