Опубликовано
2006-01-15
На SciPeople2009-08-02
ЖурналAnimal Genetics. 2006. V. 37. P. 253-257.
Gene expression profiling of hereditary exencephaly in chicken
Jaszczak K., Sazanov A.A., Sacharczuk M., Korczak M., Sazanova A.L., Parada R., Malewski T.
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Алексей Сазанов
Аннотация
In this preliminary study, differentially expressed genes were investigated in cranial tissues
from chickens with hereditary exencephaly using cDNA microarrays containing 1152
genes and expressed sequence tags (ESTs). Genes showing twofold or greater differences at
P < 0.05 between affected and normal cranial cells were considered to be candidates for
hereditary exencephaly in chicken. Eighteen ESTs (11 known genes/homologues) were
upregulated and 108 ESTs (51 known genes/homologues) were downregulated. The EST
AL584231 (ROS006C9), orthologous to human MTHFD1, a known candidate gene for
human neural tube defects (NTDs), was expressed at the same level both in normal and
affected chicken cranial tissues. ESTs AL584253 (ROS006F7, thioredoxin reductase 1) and
AL585511 (ROS024H9, thioredoxin), both involved in NTD pathogenic pathways in mice,
were downregulated and had mean ratios of 0.41 and 0.04 for expression in affected vs.
normal cells respectively. Expression differences of these two ESTs were confirmed by
quantitative real-time polymerase chain reaction. These data indicate that ESTs AL584253
and AL585511 are candidates for hereditary exencephaly in chickens.
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