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Опубликовано
2008-05-00
Опубликовано на SciPeople2009-10-15 15:22:54
ОрганизацияИнститут общей генетики им. Н.И. Вавилова РАН
ЖурналGenetika
Molecular analysis of some genes from plasmid p19 of the soil strain Bacillus subtilis 19 involved in conjugation
Genetika. 2008 May;44(5):623-30
Аннотация
Two fragments of conjugative plasmid p19 (95 kb) from the soil strain Bacillus subtilis 19 were cloned and sequenced; these fragments carry genes, products of which are indispensable for the conjugative transfer. One of the fragments 4518 bp in size carries five open reading frames and their fragments (ORF1-ORF5). The protein corresponding to ORF4 is homologous to proteins from the family VirD4. Inactivation of ORF4 and ORF1 by insertional mutagenesis caused a three-to-fivefold decrease in the frequency of plasmid p19 conjugative transfer. Another 2932-bp fragment of p19 was shown to possess a rep region homologous to the rep region of plasmid pBS72 from the soil strain B. subtilis 72 and a novel ORF (ORF6); the protein corresponding to this ORF contains the HTH motif typical for DNA-binding proteins.
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