Опубликовано
1 фев 2006
На SciPeople
13 авг 2014
Журнал
Journal of Bioinformatics and Computational Biology
1
Всего
-
Сегодня
Selection of cryptic B-cell epitopes using informational analysis of protein sequenses.
Elena Svirshcevskaya, Ludmila Alekseeva, Alexei Marchenko, Sergei Benevolenski, Valentina M. Berzhec, Alexei Nekrasov
/
Алексей (alexei) Некрасов (nekrasov)
1 фев 2006
Аннотация
Sub-unit vaccines are synthetic or recombinant peptides representing T- or B-cell epitopes
of major protein antigens from a particular pathogen. Epitope selection requires
the synthesis of peptides that overlap the protein sequences and screening for the most
effective ones. In this study a new method of immunogenic peptide selection based on
the analysis of information structure of protein sequences is suggested. The analysis
of known B-cell epitope location in the information structure of Aspergillus fumigatus
proteins Asp f 2 and Asp f 3 has shown that epitopes are scattered along the sequences
of proteins for the exception of sites with Increased Degree Information Coordination
(IDIC). Based on these results peptides from different allergens such as Asp f 2, Der p 1,
and Fel d 1 were selected and produced in a recombinant form in the context of yeast virus-like particles (VLPs). Immunization of mice with VLPs containing peptides form
allergens has induced the production of IgG able to recognize full-length antigens. This
result suggests that the analysis of information structure of proteins can be used for the
selection of peptides possessing cryptic B-cell epitope activity.
Публикация
Journal of Bioinformatics and Computational Biology
Vol. 4, No. 2, (2006), pp. 389–402.
На сайте используются файлы cookies, чтобы учесть предпочтения и улучшить работу сайта.
Продолжая использовать данный сайт, Вы соглашаетесь с использованием файлов cookies.
Нет комментариев