2013
Молекулярное типирование Yersinia pestis
Платонов М.Е., Евсеева В.В., Дентовская С.В., Анисимов А.П.
Особое внимание в обзоре уделено воспроизводимым в различных лабораториях методам генотипирования Y. pestis, позволяющим дифференцировать отдельные бактериальные...
Draft genome sequences of five Yersinia pseudotuberculosis ST19 isolates and one isolate variant
Platonov M.E., Blouin Y., Evseeva V.V., Afanas'ev M.V., Pourcel C., Balakhonov S.V., Vergnaud G., Anisimov A.P.
We report the first draft genome sequences of five Yersinia pseudotuberculosis isolates of sequence type (ST) 19 and of a variant from one of the five isolates. The total length of assemblies ranged from 4,226,485 bp to 4,274,148 bp, including between 3,808 and 3,843 predicted coding sequences.
2012
Филогеография полевочьих штаммов Yersinia pestis из природных очагов Кавказа и Закавказья
Платонов М.Е., Евсеева В.В., Светоч Т.Э., Ефременко Д.В., Кузнецова И.В., Дентовская С.В., Куличенко А.Н., Анисимов А.П.
С помощью молекулярного типирования проведен сравнительный анализ 57 штаммов Y. pestis subsp. microtus bv. caucasica. Полученные результаты свидетельствуют о наличии в составе...
Липополисахарид чумного микроба Yersinia pestis: cтруктура, генетика, биологические свойства
Книрель Ю.А., Анисимов А.П.
Приведены данные о составе и строении углеводной части (олигосахарида кора) и липидного компонента (липида А) различных форм липополисахарида (ЛПС) − одного из...
Lipopolysaccharide of Yersinia pestis, the Cause of Plague: Structure, Genetics, Biological Properties
Knirel YA, Anisimov AP
The present review summarizes data pertaining to the composition and structure of the carbohydrate moiety (core oligosaccharide) and lipid component (lipid A) of the various forms of lipopolysaccharide (LPS), one of the major pathogenicity factors ofYersinia pestis, the cause of plague. The...
2011
Определение экспрессии маркера ранней активации CD69 на лимфоцитах иммунных мышей после стимуляции их антигенами чумного микроба
Фирстова В.В., Бахтеева И.В., Титарева Г.М., Зырина Е.В., Иванов С.А., Киселева Н.В., Копылов П.Х., Анисимов А.П., Дятлов И.А.
В работе дана оценка способности антигенов чумного микроба F1 и V специфически активировать в системе in vitro субпопуляции Т-лимфоцитов мышей линии BALB/c,...
2010
Temperature-induced changes in the lipopolysaccharide of Yersinia pestis affect plasminogen activation by the pla surface protease
Suomalainen M, Lobo LA, Brandenburg K, Lindner B, Virkola R, Knirel YA, Anisimov AP, Holst O, Korhonen TK
The Pla surface protease of Yersinia pestis activates human plasminogen and is a central virulence factor in bubonic and pneumonic plague. Pla is a transmembrane beta-barrel protein and member of the omptin family of outer membrane proteases which require bound lipopolysaccharide (LPS) to be...
Факторы, обеспечивающие блокообразующую активность Yersinia pestis
Анисимов А.П.
В обзоре кратко представлены литературные данные о специфическом механизме передачи Y. pestis "блоки-рованными" блохами. Основное внимание уделено обсуждению...
Amino acid and structural variability of Yersinia pestis LcrV protein
Anisimov AP, Dentovskaya SV, Panfertsev EA, Svetoch TE, Kopylov PKh, Segelke BW, Zemla A, Telepnev MV, Motin VL
The LcrV protein is a multifunctional virulence factor and protective antigen of the plague bacterium and is generally conserved between the epidemic strains of Yersinia pestis. We investigated the diversity in the LcrV sequences among non-epidemic Y. pestis strains which have a limited virulence...
2009
Tetraacylated lipopolysaccharide of Yersinia pestis can inhibit multiple Toll-like receptor-mediated signaling pathways in human dendritic cells
Telepnev MV, Klimpel GR, Haithcoat J, Knirel YA, Anisimov AP, Motin VL
BACKGROUND: Yersinia pestis, the causative agent of plague, showed a temperature-dependent change in lipid A composition, with a reduced degree of acylation when bacteria were grown at 37 degrees C (tetraacylated) versus ambient temperature (hexaacylated).
METHODS: Human monocytes and...
Revision of the O-polysaccharide structure of Yersinia pseudotuberculosis O:1b
Kondakova AN, Shaikhutdinova RZ, Ivanov SA, Dentovskaya SV, Shashkov AS, Anisimov AP, Knirel YA
The O-polysaccharide of Yersinia pseudotuberculosis O:1b was reinvestigated using (1)H and (13)C NMR spectroscopy, and the anomeric configuration of the mannose residue at the branching point was revised. The following is the revised structure of the O-polysaccharide: [structure: see the text]...
Genotyping and phylogenetic analysis of Yersinia pestis by MLVA: insights into the worldwide expansion of Central Asia plague foci
Li Y, Cui Y, Hauck Y, Platonov ME, Dai E, Song Y, Guo Z, Pourcel C, Dentovskaya SV, Anisimov AP, Yang R, Vergnaud G
BACKGROUND: The species Yersinia pestis is commonly divided into three classical biovars, Antiqua, Medievalis, and Orientalis, belonging to subspecies pestis pathogenic for human and the (atypical) non-human pathogenic biovar Microtus (alias Pestoides) including several non-pestis subspecies....
Pleiotropic effects of the lpxM mutation in Yersinia pestis resulting in modification of the biosynthesis of major immunoreactive antigens
Feodorova VA, Pan'kina LN, Savostina EP, Kuznetsov OS, Konnov NP, Sayapina LV, Dentovskaya SV, Shaikhutdinova RZ, Ageev SA, Lindner B, Kondakova AN, Bystrova OV, Kocharova NA, Senchenkova SN, Holst O, Pier GB, Knirel YA, Anisimov AP, Motin VL
Deletion mutants in the lpxM gene in two Yersinia pestis strains, the live Russian vaccine strain EV NIIEG and a fully virulent strain, 231, synthesise a less toxic penta-acylated lipopolysaccharide (LPS). Analysis of these mutants revealed they possessed marked reductions in expression and...
Structure of the O-antigen of Yersinia pseudotuberculosis O:4a revised
Kondakova AN, Bystrova OV, Shaikhutdinova RZ, Ivanov SA, Dentovskaya SV, Shashkov AS, Knirel YA, Anisimov AP
The O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Yersinia pseudotuberculosis O:4a and studied by NMR spectroscopy, including 2D ROESY and (1)H, (13)C HMBC experiments. The following structure of the pentasaccharide repeating unit of the...
Structure of the O-polysaccharide of Yersinia pseudotuberculosis O:2b
Kondakova AN, Bystrova OV, Shaikhutdinova RZ, Ivanov SA, Dentovskaya SV, Shashkov AS, Knirel YA, Anisimov AP
The O-polysaccharide was isolated by hydrolysis of the lipopolysaccharide of Yersinia pseudotuberculosis O:2b, and studied by 1D and 2D NMR spectroscopy. The following structure of the polysaccharide was established: alpha-Abep. 1-->3. -->2)...
Structure of the O-antigen of Yersinia pseudotuberculosis O:4b
Kondakova AN, Bystrova OV, Shaikhutdinova RZ, Ivanov SA, Dentovskaya SV, Shashkov AS, Knirel YA, Anisimov AP
Structures of the O-antigens of Yersinia pseudotuberculosis O2c and O3 were reinvestigated by NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC, and HMBC experiments. The following revised structure of the O2c tetrasaccharide repeating unit was established, which...
The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice
Anisimov AP, Bakhteeva IV, Panfertsev EA, Svetoch TE, Kravchenko TB, Platonov ME, Titareva GM, Kombarova TI, Ivanov SA, Rakin AV, Amoako KK, Dentovskaya SV
Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and...
2008
Reinvestigation of the O-antigens of Yersinia pseudotuberculosis: revision of the O2c and confirmation of the O3 antigen structures
Kondakova AN, Bystrova OV, Shaikhutdinova RZ, Ivanov SA, Dentovskaya SV, Shashkov AS, Knirel YA, Anisimov AP
Structures of the O-antigens of Yersinia pseudotuberculosis O2c and O3 were reinvestigated by NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC, and HMBC experiments. The following revised structure of the O2c tetrasaccharide repeating unit was established, which...
Insight into Microevolution of Yersinia pestis by Clustered Regularly Interspaced Short Palindromic Repeats
Yujun Cui, Yanjun Li, Olivier Gorgé, Mikhail E. Platonov, Yanfeng Yan, Zhaobiao Guo, Christine Pourcel, Svetlana V. Dentovskaya, Sergey V. Balakhonov, Xiaoyi Wang, Yajun Song, Andrey P. Anisimov, Gilles Vergnaud, Ruifu Yang
Background
Yersinia pestis, the pathogen of plague, has greatly influenced human history on a global scale. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR), an element participating in immunity against phages' invasion, is composed of short repeated sequences separated by...
2006
Конструирование вакцинных штаммов грамотрицательных бактерий со сниженной реактогенностью
Дентовская С.В., Шайхутдинова Р.З., Книрель Ю.А., Иванов С.А., Анисимов А.П.
Основным недостатком используемых в настоящее время живых и убитых вакцин на основе грамотрицательных бактерий является высокая реактогенность, определяемая...
Treatment of plague: promising alternatives to antibiotics
Andrey P. Anisimov, Kingsley K. Amoako
Plague still poses a significant threat to human health, and interest has been renewed recently in the possible use of Yersinia pestis as a biological weapon by terrorists. The septicaemic and pneumonic forms are always lethal if untreated. Attempts to treat this deadly disease date back to the...
2005
Cold temperature-induced modifications to the composition and structure of the lipopolysaccharide of Yersinia pestis
Yuriy A. Knirel, Buko Lindner, Evgeny Vinogradov, Rima Z. Shaikhutdinova, Sof’ya N. Senchenkova, Nina A. Kocharova, Otto Holst, Gerald B. Pier, Andrey P. Anisimov
Following a report of variations in the lipopolysaccharide (LPS) structure of Yersinia pestis at mammalian (37 °C) and flea (25 °C) temperatures, a number of changes to the LPS structure were observed when the bacterium was cultivated at a temperature of winter-hibernating rodents (6 °C). In...
Intraspecies and Temperature-Dependent Variations in Susceptibility of Yersinia pestis to the Bactericidal Action of Serum and to Polymyxin B
Andrey P. Anisimov, Svetlana V. Dentovskaya, Galina M. Titareva, Irina V. Bakhteeva, Rima Z. Shaikhutdinova, Sergey V. Balakhonov, Buko Lindner, Nina A. Kocharova, Sof'ya N. Senchenkova, Otto Holst, Gerald B. Pier, Yuriy A. Knirel
Lipopolysaccharide (LPS) structure impacts the bactericidal action of cationic peptides, such as polymyxin B (PMB), and sensitivity to killing by normal human serum (NHS). Cultivation of different subspecies strains of Yersinia pestis isolated from unrelated geographic origins at various...
Temperature-Dependent Variations and Intraspecies Diversity of the Structure of the Lipopolysaccharide of Yersinia pestis
Yuriy A. Knirel, Buko Lindner, Evgeny V. Vinogradov, Nina A. Kocharova, Sof'ya N. Senchenkova, Rima Z. Shaikhutdinova, Svetlana V. Dentovskaya, Nadezhda K. Fursova, Irina V. Bakhteeva, Galina M. Titareva, Sergey V. Balakhonov, Otto Holst, Tat'yana A. Gremyakova, Gerald B. Pier, Andrey P. Anisimov
Yersinia pestis spread throughout the Americas in the early 20th century, and it occurs predominantly as a single clone within this part of the world. However, within Eurasia and parts of Africa there is significant diversity among Y. pestis strains, which can be classified into different biovars...
2004
Intraspecific Diversity of Yersinia pestis
Andrey P. Anisimov, Luther E. Lindler, Gerald B. Pier
Increased interest in the pathogenic potential of Yersinia pestis has emerged because of the potential threats from bioterrorism. Pathogenic potential is based on genetic factors present in a population of microbes, yet most studies evaluating the role of specific genes in virulence have used a...
2002
Факторы Yersinia pestis, обеспечивающие циркуляцию и сохранение возбудителя чумы в экосистемах природных очагов. Сообщение 2
Анисимов А.П.
Для постоянной циркуляции в природных очагах возбудитель чумы должен проникнуть в организм хозяина, противодействовать защитным бактерицидным системам...
Факторы Yersinia pestis, обеспечивающие циркуляцию и сохранение возбудителя чумы в экосистемах природных очагов. Сообщение 1
Анисимов А.П.
Для постоянной циркуляции в природных очагах возбудитель чумы должен проник-нуть в организм хозяина, противодействовать защитным бактерицидным системам...
The core structure of the lipopolysaccharide from the causative agent of plague, Yersinia pestis.
Vinogradov E.V., Lindner B., Kocharova N.A., Senchenkova S.N., Shashkov A.S., Knirel Yu.A., Holst O., Gremya-kova T.A., Shaikhutdinova R.Z., Anisimov A.P.
The rough-type lipopolysaccharide (LPS) of the plague pathogen, Yersinia pestis, was studied after mild-acid and strong-alkaline degradations by chemical analyses, NMR spectroscopy and electrospray-ionization mass spectrometry, and the following structure of the core region was determined:where...
2000
1999
Молекулярно-генетические механизмы образования и функциональная значимость капсулы Yersinia pestis
Анисимов А.П.
Диссертация посвящена получению новых сведений о молекулярно-генетических механизмах образования капсулы Y. pestis, ее функциональной значимости в патогенезе и...
1997
A novel mechanism of antibiotic resistance in plague?
Anisimov A.P., Dyatlov I.A.
1996
Virulence of pPst+ and pPst- strains of Yersinia pestis for guinea-pigs
Samoilova S.V., Samoilova L.V., Yezhov I.N., Drozdov I.G., Anisimov A.P.
Guinea-pigs were infected subcutaneously or by respiratory challenge with plasmid-containing (pPst+pCad+pFra+) Yersinia pestis strain 358 and its pPst-pCad+pFra+, pPst+pCad+pFra- and pPst- pCad+pFra- derivatives, grown in vitro at 28°C or at 37°C. Lack of plasmid pPst did not lead to an...
1995
Virulent non-capsulate Yersinia pestis variants constructed by insertion mutagenesis
Drozdov I.G., Anisimov A.P., Samoilova S.V., Yezhov I.N., Yeremin S.A., Karlyshev A.V., Krasilnikova V.M., Kravchenko V.I.
Insertion mutagenesis with the help of the plasmid pFS23 was used to generate Yersinia pestis fra mutants. The results of pFra- strain production under non-selective conditions suggested that such Y. pestis variants may be generated in natural plague foci at high frequency and may participate in...
1993
Физико-химическая и биологическая характеристика рН6-антигена Yersinia pestis выделенного иммуносорбционным методом
Степаншина В.Н., Гремякова Т.А., Анисимов А.П., Потапов В.Д.
Способ получения бескапсульных вариантов возбудителя чумы
Дроздов И.Г., Ежов И.Н., Самойлова С.В., Анисимов А.П., Карлышев А.В., Кравченко В.И., Красильникова В.М., Еремин С.А., Никифоров А.К.
Сконструирован гибридный репликой pFS23, содержащий функционально неактивный участок fra-оперона pFra Yersinia pestis EV и способный к гомологичной рекомбинации с плазмидой...
Оценка биологических свойств бескапсульных вариантов возбудителя чумы
Дроздов И.Г., Ежов И.Н., Самойлова С.В., Анисимов А.П., Никифоров А.К.
Коллекция штаммов чумного микроба, утративших способность синтезировать видоспецифический капсульный антиген (ФI), охарактеризована по основным видовым...
1991
Получение авирулентных вариантов Yersinia pestis c Fra+, V+, Cad+, Fib-Coa+, PstI+, Psb+ фенотипом
Анисимов А.П., Карлышев А.В., Кравченко В.И., Красильникова В.М., Кокушкин А.М.
Конструирование рекомбинантных плазмид, стабильно наследующихся в клетках Yersinia pestis
Анисимов А.П., Карлышев А.В., Павлов В.М., Кравченко В.И.