О проекте

A range of plant characters are currently available for distinguishing between closely related individuals. RFLP (restriction fragment length polymorphism), which is widely used for gene mapping and studying diversity in plant populations. RFLP analysis uses hybridization technology; cloned DNA sequences are labelled (usually radioactively) and used as probes to identify differences in the sizes of specific genomic DNA fragments following digestion by a restriction endonuclease. The technique PCR-sequencing is used for studying variation in this case are base sequences of known genes. The polymerase chain reaction is utilized here in order to amplify DNA between 20-base specific primers, and this amplified DNA is then used for sequencing. The technique reveals variation at a very high level of resolution, since differences are measured at the base level. RAPD (random amplified polymorphic DNA) which offers advantages in speed, technical simplicity and the frequency of identification of polymorphisms.

P Molecular Studies Of Genomic Dna Isolated From Antimalarial Plants- By Dr. U. Lakshmi Narasaiah, and Jayaprada

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