Ирина Концевая » Публикация

Аннотация The Russian Federation is a high-tuberculosis burden country with high rates of multidrug and extensive drug resistance (M/XDRTB) especially in HIV co-infected patients. Rapid and reliable diagnosis for detection of resistance to second-line drugs is vital for adequate patient management.We evaluated the performance of the GenoType® MTBDRsl (Hain Lifescience GmbH, Nehren, Germany) assay on smear-positive sputum specimens obtained from 90 HIV-infected MDR TB patients from Russia. Test interpretability was over 98%. Specificity was over 86% for all drugs while sensitivity varied being the highest (71.4%) for capreomycin and lowest (9.4%) for kanamycin probably due to presence of mutations in the eis gene. The sensitivity to detect XDRTB was 13.6% increasing to 42.9% if kanamycin (not commonly used in Western Europe) was excluded. The assay is a highly specific screening tool for XDR detection in direct specimens from HIV co-infected TB patients, but cannot be used to rule out XDR TB.